Study SRP033492 Download

TitleGSE52964: BAF155 methylation at R1064 creates unique chromatin association patterns
AbstractSummary: CARM1, a coactivator for various cancer-relevant transcription factors, is overexpressed in breast cancer. To elucidate the functions of CARM1 in tumorigenesis, we knocked out CARM1 from several breast cancer cell lines using Zinc-Finger Nuclease technology, which resulted in drastic phenotypic and biochemical changes. The CARM1 KO cell lines enabled identification of novel CARM1 substrates, notably the SWI/SNF core subunit BAF155. Methylation of BAF155 at R1064 was found to be an independent prognostic biomarker for cancer recurrence and to regulate breast cancer cell migration and metastasis. Further, CARM1-mediated BAF155 methylation affects gene expression by directing methylated BAF155 to unique chromatin regions (e.g., c-Myc pathway genes). Collectively our studies uncover a mechanism by which BAF155 acquires tumorigenic functions via arginine methylation. Overall Design: Examination of methylation of BAF155 (R1064) in breast cancer cells
Referencesno content
OrganismsHomo sapiens
TypeEpigenetics
Descriptionno content
SubmitterGene Expression Omnibus
Related objects
SubmissionsSRA115254
LinksGEO Series GSE52964, Unknown DB(gds)
GEO Accession GSE52964
Project Contact Name = Lu, Wang; Email = wanglunju@gmail.com; Department = Oncology; Institute = UW madison; Address = 1400 university ave; City = madison; Zip/Postal Code = 53705; Country = USA
Project Contributor Lu, Wang, Mark, Meyer, J, W, Pike, Wei, Xu