Study SRP012558 Download

TitlePreborn infant gut metagenome - Carrol stool sample
AbstractThe gastrointestinal microbiome undergoes shifts in species and strain abundances, yet dynamics involving closely related microorganisms remain largely unknown because most methods cannot resolve them. We utilized new metagenomic methods for tracking microbial communities in 11 fecal samples collected during the first month of life of a premature infant. 96 % of the sequencing reads were assembled into scaffolds of >500 bp length that could be assigned to organisms at the strain level. Six essentially complete (~99 %) and two near-complete genomes were assembled for bacteria that comprised as little as 1 % of the community, as well as nine partial genomes representing as little as 0.05 %. In addition, three viral genomes were assembled and assigned to their hosts. These results enabled a comprehensive study of species and strain level variations in the community. The relative abundance of three Staphylococcus epidermidis strains, as well as three phage that infect them, changed dramatically over time. Genes possibly related to these shifts include those for resistance to antibiotics, heavy metals and phage. At the species level we observed the decline of an early-colonizing Propionibacterium acnes strain similar to SK137 and the proliferation of novel Propionibacterium and Peptoniphilus species late in colonization. The Propionibacterium species differed in their ability to metabolize carbon compounds such inositol and sialic acid, indicating that shifts in species composition likely impact the metabolic potential of the community. These results highlight the benefit of reconstructing complete genomes from metagenomic data and demonstrate methods for achieving this goal.
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Organismshuman gut metagenome
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SubmitterUniversity of California, Berkeley
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