Sample SRS282747 Download

TitleSequence_Capture_of_25-36
Anonymized nameno content
Scientific nameGossypium hirsutum
Common nameno content
DescriptionWhole genome DNA samples were extracted using a modified CTAB protocol. Twelve samples were tagged with multiplex identifiers (MIDs), each with a different MID. They were idependently hybridized to a custom Nimblegen Sequence Capture Array (12-plex). After washing, the samples were all eluted into a single tube and subjected to ligation-mediated PCR (i.e. PCR using the 454 sequence adapters) to amplify the captured fragments to a high enough concentration for sequencing. Subsequently, the libraries were prepared for 454 sequencing (size selection on a Capliper XL, emPCR, and bead preparation etc.). The twelve captured samples tagged with multiplex identifiers (MIDs) were run on a single plate (2 large regions) of 454. The samples were prepared using 'traditional' WGS libraries from Roche. Below is a list of the samples and MIDs included in this run. Sample Name MID MID Forward Sequence (in that order). TX_2002 1 ACGAGTGCGT; TX_2090 2 ACGCTCGACA; TX_2091 3 AGACGCACTC; TX_2092 4 AGCACTGTAG; TX_2093 5 ATCAGACACG; TX_2094 6 ATATCGCGAG; TX_2095 7 CGTGTCTCTA; TX_2096 8 CTCGCGTGTC; ARK_2402 9 TAGTATCAGC; ST825 10 TCTCTATGCG; TX_1988 11 TGATACGTCT; DP90 12 TACTGAGCTA;
Referencesno content
OrganismGossypium hirsutum
Cell typeSRS282747
SubmitterBrigham Young University DNA Sequencing Center -Udall Lab
Related objects
SubmissionsSRA048701
LinksUnknown DB(biosample)