Sample SRS282740 Download

TitleSequence_Capture_of_13-24
Anonymized nameno content
Scientific nameGossypium hirsutum
Common nameno content
DescriptionWhole genome DNA samples were extracted using a modified CTAB protocol. Twelve samples were tagged with multiplex identifiers (MIDs), each with a different MID. They were idependently hybridized to a custom Nimblegen Sequence Capture Array (12-plex). After washing, the samples were all eluted into a single tube and subjected to ligation-mediated PCR (i.e. PCR using the 454 sequence adapters) to amplify the captured fragments to a high enough concentration for sequencing. Subsequently, the libraries were prepared for 454 sequencing (size selection on a Capliper XL, emPCR, and bead preparation etc.). The twelve captured samples tagged with multiplex identifiers (MIDs) were run on a single plate (2 large regions) of 454. The samples were prepared using 'traditional' WGS libraries from Roche. Below is a list of the samples and MIDs included in this run. Sample Name MID MID Forward Sequence (in that order) Sample Name MID MID Forward Sequence TX_1182 1 ACGAGTGCGT; TX_44 2 ACGCTCGACA; TX_959 3 AGACGCACTC; TX_1110 4 AGCACTGTAG; TX_1120 5 ATCAGACACG; TX_1226 6 ATATCGCGAG; TX_1228 7 CGTGTCTCTA; TX_674 8 CTCGCGTGTC; TX_1046 9 TAGTATCAGC; TX_1982 10 TCTCTATGCG; TX_1236 11 TGATACGTCT; TX_1996 12 TACTGAGCTA;
Referencesno content
OrganismGossypium hirsutum
Cell typeSRS282740
SubmitterBrigham Young University DNA Sequencing Center -Udall Lab
Related objects
SubmissionsSRA048701
LinksUnknown DB(biosample)