Run SRR921528 Download

InstrumentIllumina Genome Analyzer IIx
Instrument nameno content
Experiment aliasGSM1173494
Referencesno content
OrganismMus musculus
SubmitterGene Expression Omnibus
Library nameno content
Library strategyOTHER
Library sourceGENOMIC
Library selectionother
Library layoutPAIRED
Library construction protocolSingle cells treated according to the modified Hi-C protocol were isolated in PBS and incubated at 65 degrees C for overnight. The biotin-labelled DNA from each cell was bound to Dynabeads M-280 streptavidin magnetic beads (Life Technologies), digested with Alu I (New England Biolabs), A-tailed with Klenow Fragment (3'->5' exo-) (New England Biolabs) and dATP, ligated with customised Illumina adapter with T4 DNA ligase (New England Biolabs), then the library was amplified by PCR with library amplification primers, and size-selected between 300 and 700 bp on an agarose gel.
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