Run SRR1032898 Download

CenterGEO
InstrumentIllumina HiSeq 2000
Instrument nameno content
AliasGSM1269368_r1
Experiment aliasGSM1269368
Referencesno content
OrganismSchizosaccharomyces pombe
SubmitterGene Expression Omnibus
Library nameno content
Library strategyRNA-Seq
Library sourceTRANSCRIPTOMIC
Library selectionsize fractionation
Library layoutSINGLE
Library construction protocolRNA was extracted using the hot phenol method (Leeds et al., 1991), and small RNAs were purified using the mirVanaTM miRNA Isolation kit (Ambion). These sRNAs were used for library construction. Small RNA libraries were constructed as previously described (Halic and Moazed, 2010). Briefly, 20-30nt RNA was size-selected on a 17.5% polyacrylamide/7M urea gel and ligated to a 3’ adapter. The ligated species were size-selected on a 17.5% polyacrylamide/7M urea gel and ligated to a 5’ adapter. RNA was then reverse transcribed into cDNA and PCR-amplified in a two-step process. Amplified cDNA was gel-purified and sequenced on Illumina High-Seq 2000 platform.
Related objects
SubmissionsSRA111633
Linksno content