Experiment SRX387003 Download

TitleGSM1279587: wild-type short footprints; Saccharomyces cerevisiae; OTHER
AliasGSM1279587
Design descriptionno content
Referencesno content
OrganismsSaccharomyces cerevisiae
SubmitterGene Expression Omnibus
InstrumentIllumina HiSeq 2500
Library nameno content
Library strategyOTHER
Library sourceOTHER
Library selectionother
Library layoutSINGLE
Library construction protocolRibosome profiling lysates were clarified, RNase treated, and run over sucrose gradients to extract the monosome or disome peak. RNA was then extracted using SDS/hot phenol/chloroform and footprints of the specified size were retained by gel purification. Small footprints of 15-24 nt were retained during RNA size selection. These were later separated into 15-18 and 19-24 nt pools during data processing. Briefly, RNAs were ligated to a universal DNA linker followed by reverse transcription. cDNAs were then circularized and PCR amplified. Ribosome profiling circularized cDNA was subject to ribosomal RNA subtraction using custom biotinylated oligos.
Related objects
SubmissionsSRA115268
LinksGEO Sample GSM1279587, Unknown DB(gds)
GEO Accession GSM1279587