Experiment SRX373673 Download

TitleGSM1260066: Ter119+ poly(A)- RNA; Mus musculus; RNA-Seq
Design descriptionno content
Referencesno content
OrganismsMus musculus
SubmitterGene Expression Omnibus
InstrumentIllumina HiSeq 2000
Library nameno content
Library strategyRNA-Seq
Library selectioncDNA
Library layoutPAIRED
Library construction protocolE14.5 mouse fetal liver cells were separated into TER119+ and TER119- fractions via magnetic-assisted cell sorting. Total RNA was isolated from these cells using the QIAGEN miRNeasy Kit according to the manufacturer’s instructions. Ribosomal RNA was depleted from 4 ug total RNA using the Ribo-Zero Gold Kit from Epicentre. Strand-specific sequencing libraries were generated following a previously described protocol (PMID: 21943893) from the total RNA (TER119- and TER119+ cells) or from the poly(A)+ and poly(A)- fractions (TER119+ cells). The latter fractions were separated using the Solexa kit (Illumina) according to the manufacturer’s instructions. cDNA fragments of 400-600 bp from these libraries were selected by gel purification and then sequenced on a Illumina HiSeq2000 sequencer.
Related objects
LinksGEO Sample GSM1260066, Unknown DB(gds)
GEO Accession GSM1260066