Experiment ERX358060 Download

TitleApplication of Histone Interacting Domains as an Alternative to Antibodies (CIDOP-seq)
AliasE-MTAB-2143:4_Dnmt3a_i78_filtered.fastq.gz
Design descriptionApplication of Histone Interacting Domains as an Alternative to Antibodies (CIDOP-seq)
Referencesno content
OrganismsHomo sapiens
SubmitterUniversity of Stuttgart
InstrumentIllumina HiSeq 2000
Library nameDnmt3a PWWP
Library strategyOTHER
Library sourceGENOMIC
Library selectionother
Library layoutSINGLE
Library construction protocolHepG2 cells were grown following ENCODE guidelines Chromatin IP kit (Active Motif) 15-60 micrograms of mononucleosomes were incubated overnight with HMIDs and were washed 1x High Salt Buffer, 1x Low Salt Buffer, 1x LiCl Buffer and 2x TE buffer, eluted in 1% SDS and DNA was extracted with Chromatin IP kit (Active Motif)
Related objects
SubmissionsERA275354
Linksno content
Experimental Factor: reading domain Dnmt3a PWWP