Experiment ERX009442 Download

TitleSequencing of Wheat chromosome 1D short arm within the GABI-BARLEX project
Design descriptionIndividual wheat chromosome arms were isolated by flow sorting and shotgun sequenced by 454 Titanium pyrosequencing.
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OrganismsTriticum aestivum
Instrument454 GS FLX Titanium
Library nameWCAChineseSpring
Library strategyWCS
Library sourceGENOMIC
Library selectionother
Library layoutSINGLE
Library construction protocolIntact mitotic chromosomes were isolated by flow cytometric sorting and the purity of the obtained chromosome suspension was determined by FISH essentially as described previously (Suchankova et al., 2006). The DNA of sorted chromosomes was purified and amplified by MDA as described by Simkova et al. (2008).
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