Experiment ERX009426 Download

TitleSequencing of Barley chromosome 2 short arm within the GABI-BARLEX project
AliasWCA2HSBetzes
Design descriptionIndividual barley chromosomes and chromosome arms were isolated by flow sorting and shotgun sequenced by 454 Titanium pyrosequencing.
Referencesno content
OrganismsHordeum vulgare subsp. vulgare
SubmitterIPK-Gatersleben
Instrument454 GS FLX Titanium
Library nameWCABetzes
Library strategyWCS
Library sourceGENOMIC
Library selectionother
Library layoutSINGLE
Library construction protocolIntact mitotic chromosomes were isolated by flow cytometric sorting and the purity of the obtained chromosome suspension was determined by FISH essentially as described previously (Suchankova et al., 2006). The DNA of sorted chromosomes was purified and amplified by MDA as described by Simkova et al. (2008).
Related objects
SubmissionsERA015647
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